Confocal Laser Scanning Microscope
The new Olympus FluoView™ FV1000 is the latest in point-scanning, point-detection, confocal laser scanning microscopes designed for today's intensive and demanding biological research investigations. Excellent resolution, bright and crisp optics, and high efficiency of excitation, coupled to an intuitive user interface and affordability are key characteristics of this state-of-the-art optical microscopy system. Our system has four simultaneous detection channels, including one for DIC and two spectral channels. Source.
Need help deciding which fluorophore to use? Click here to open a detailed Invitrogen Alexa Fluor guide.
Available Laser Lines:
|10X NA 0.40||UPLanSAPO||Air|
|20X NA 0.75||UPLanSAPO||Air|
|40X NA 0.90||UPLanSAPO||Air|
|40X NA 1.30||UPLanFLN||Oil|
|60X NA 1.42||PLanAPO||Oil|
|100X NA 1.40||UPlanSAPO||Oil|
Figure 1: Confocal miscroscope images of autofluorescence in pond algae found in Pocatello, Idaho (left) and Ascomycotes sp., cup fungus (right). Two different emission wavelengths are detected in each sample.
Figure 2: Paramecium, A) Projected z-stack of fluorescence images of cilia structures of multiple microorganisms. B) Differential interference contrast (DIC) image of the same group of paramecium. Both images were captured simultaneously on the confocal laser scanning microscope.
Figure 3: Autofluorescence in Pinus laricio. The confocal laser scanning microscope was used to acquire a lambda scan, which samples a user-defined range of wavelengths of fluorescence emission. Lambda scans are useful for identifying peak fluorescent wavelengths when using uncertain fluorophores and for identifying autofluorescence in samples.